Previous studies suggest that maternal obesity throughout gestation can program these diseases in offspring throughout life. We tested the effects of maternal obesity on 9-11-year-old (aged) offspring through a 12-week ad libitum feeding trial and a tissue analyses following necropsy 2 years later. We hypothesized that after finishing an ad-libitum feeding trial, OBF1 would present increased fat content, cortisol concentrations and insulin resistance. Weights did not differ by treatment group, however, post feeding trial fat percentage (p=0.03) and fat content in grams (p=0.03) was increased in OBF1 ewes. Baseline and necropsy blood samples showed an increase in cortisol (p=.05) and insulin concentration (p=.05) but no difference in glucose concentration (p>0.1). Increase in Insulin concentration with no increase in glucose concentration suggest that the OBF1 ewes experienced insulin resistance which is a key characteristic in diabetes mellitus. In the Left Ventricle (LV) of OBF1, we found a significant increase in superoxide dismutase 1 (P=0.03) and 2 (p=0.009), catalase (P<.001), glutathione peroxidase (p=0.03), interleukin-6 (p=0.005), and tumor necrosis factor α (p=0.01) compared to control offspring (CONF1). In the left lobe of the liver of OBF1, however, there was a significant decrease in GPx (p=0.02), and TNFα (p=0.02) compared to the CONF1. As a result of the observed increase in cortisol concentration and insulin resistance, we would hypothesize that in future investigations we will see increased concentration of inflammatory proteins associated with increased cortisol, insulin, and adipose tissue IL-6, NFκB, and TNFα as well as an increase in oxidative stress. This evidence suggests that the adverse effects we have seen in young OBF1 lasts into elderly age. The results in the liver and LV suggests there are separate mechanisms involving inflammation and oxidative stress between organs.
|Commitee:||Nathanielsz, Peter, Navratil, Amy|
|School:||University of Wyoming|
|School Location:||United States -- Wyoming|
|Source:||MAI 81/4(E), Masters Abstracts International|
|Subjects:||Aquatic sciences, Aging|
|Keywords:||Aging, Inflammation, Maternal Obesity, Oxidative Stress|
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