Aptamers like ssDNA and RNA have a very high affinity towards their target. RNA being a naturally flexible nucleic acid can form a variety of structures and is capable of binding to a particular target in multiple ways. This makes RNA a better aptamer. SELEX (Systematic Evolution of Ligand by Exponential Enrichment) is a method for identification of aptamers. Cell-SELEX uses whole cells as the target. A novel method for the DNA aptamer identification was recently developed in our lab using Surface Plasmon Resonance (SPR) to accelerate the process of Cell-SELEX. This research has expanded to the optimization of a round of SPR-Cell-SELEX for RNA using the target E. coli K12. The optimization has been performed for steps involved like PCR, in vitro transcription, and SPR parameters like flow rate, library concentration, and elution buffer. The gel analysis of the DNA library after the first round of selection appeared to be shorter than the initial DNA library by a few bases, not affecting the further rounds of selection.
|Commitee:||Voss, Eric J., De Meo, Cristina, Shavezipur, Kamran|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 81/3(E), Masters Abstracts International|
|Keywords:||Aptamer, SELEX, Surface plasmon resonance|
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