Geminiviruses are the largest family of plant viruses and cause some of the most economically important diseases of crops. Beet curly top virus (BCTV) is the type species of the genus Curtovirus. BCTV affects more than 300 plant species and causes curly top disease in several economically important crops such as pepper, common bean, sugar beet, tomato and spinach. Curly top disease can be a major constraint of pepper production in the western and southern United States as well as northcentral and western Mexico where the disease has been documented for over a century. BCTV infection in pepper can result in yield losses ranging from 17-48%, depending on the stage of growth plants are infected. Breeding for resistance to BCTV is complicated by the diversity of BCTV strains and their prevalence, virulence, and host specificity. Only a few poorly characterized potential sources of moderate resistance to BCTV have been reported by researchers at New Mexico State University based on screening of various germplasm collections. In the present study, agroinoculation and beet leafhopper transmission methods were used to identify resistant wild pepper germplasm. Pepper F2 populations were systematically screened and genetically characterized for BCTV resistance against an isolate of the pepper-infecting BCTV strain BCTV-Pepper curly top [US-Cal-BV3-Tom-09] (BCTV-PeCT-BV3). Disease screening of three populations indicated a two-loci model for the resistance. Bulk segregant analysis of genotyping-by-sequencing data from one of these resistant populations indicated five QTL linked to the resistance trait. Identification and introgression of genetic resistance to BCTV from wild pepper accessions to cultivated varieties would benefit crop producers and the environment through reduced pesticide applications and costs, and reduced crop loss.
|Advisor:||Van Deynze, Allen, Gilbertson, Robert L.|
|Commitee:||Walker, M. Andrew|
|School:||University of California, Davis|
|Department:||Horticulture and Agronomy|
|School Location:||United States -- California|
|Source:||DAI-B 81/2(E), Dissertation Abstracts International|
|Subjects:||Plant sciences, Plant Pathology, Genetics|
|Keywords:||Agroinoculation, Beet curly top virus, Bulk segregant analysis, Capsicum annuum, Germplasm, Quantitative trait loci|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be