Dissertation/Thesis Abstract

Cytotoxicity of Caffeine on MCF-7 Cells Measured by XTT Cell Proliferation Assay and Caffeine Intake Recommendations after Breast Cancer Diagnosis
by Collier, Machell, M.S.N., Bastyr University, 2019, 56; 13900847
Abstract (Summary)

Objectives: Caffeine is a phytochemical naturally occurring in coffee and tea. Research suggests MCF-7 breast cancer cell viability decreases after exposure to millimolar (mM) amounts of caffeine in vitro. The effect of smaller amounts has not been fully investigated. In addition, caffeine and the risk for developing breast cancer has been investigated, but less research has addressed this relationship after a diagnosis. This study assessed the impact of micromolar (µM) concentrations of caffeine on MCF-7 cell proliferation, and gathered caffeine intake recommendations given to patients with breast cancer to evaluate whether these recommendations were based on current research.

Methods: MCF-7 cells were cultured for 24 hours with caffeine concentrations following a dilution curve from 10µM to 1.2mM, and 5mM. An XTT assay calculated cell proliferation. A REDCap online survey submitted to breast cancer support groups and social media sites gathered caffeine data from patients with breast cancer.

Results: XTT absorbance data combined from all three runs indicated differences in proliferation significance at 5mM caffeine concentration (p<0.001) compared to baseline (0µM of caffeine); however, the first run alone suggested significance at multiple caffeine concentrations including at 320µM (p=0.009), 640µM (p<0.001), and 1.2mM (p<0.001). Regarding survey results, of 40 survey participants, 38 received no caffeine recommendations from healthcare providers, 36 received no information from other sources regarding caffeine related to their diagnosis, and 30 indicated they drank caffeinated coffee, tea or soda daily.

Conclusions: This data reflects previous research indicating caffeine is cytotoxic to MCF-7 cells in vitro at 5mM, and further suggests micromolar concentrations of caffeine have a bimodal effect with potential initial proliferation increase, then increasing cytotoxic effect. The in vitro data suggests possible implications regarding caffeine intake and breast cancer cell cytotoxicity, while survey data indicates absence of caffeine recommendations after diagnosis.

Indexing (document details)
Advisor: Kazaks, Alexandra, Conley, Melissa
Commitee: Helsel, Diane, Messner, Don
School: Bastyr University
Department: Department of Nutrition
School Location: United States -- Washington
Source: MAI 81/3(E), Masters Abstracts International
Subjects: Nutrition, Oncology
Keywords: Breast cancer, Caffeine, MCF-7
Publication Number: 13900847
ISBN: 9781088331538
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