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Dissertation/Thesis Abstract

Analysis and Development of Management Tools for Oryctes rhinoceros (Coleoptera: Scarabaeidae)
by Adams, Brandi-Leigh H., M.S., University of Hawai'i at Manoa, 2019, 103; 13865466
Abstract (Summary)

Oryctes rhinoceros (Coleoptera: Scarabaeidae) is a pestiferous beetle causing severe damage to coconut and other palms by boring into and feeding upon the crown tissue. Several tools are used to manage this beetle, including pheromone lured traps and the biocontrol agent, Oryctes rhinoceros nudivirus (OrNV). The pheromone lure, ethyl 4-methyloctanoate, was assessed for its attractive ability towards the CRB-S and CRB-G haplotypes by comparing the distribution of the haplotypes in non trap-caught and trap-caught populations collected from Palau. A diagnostic qPCR assay was designed to detect OrNV, and was found to be a more sensitive and accurate method for OrNV detection compared to the conventional PCR assay. Finally, because the newly emerged CRB-G haplotype has acquired resistance to OrNV, RNA extractions were sent for high-throughput sequencing to discover other viruses present in O. rhinoceros. Three sequences were discovered to be similar in sequence to viruses of other insect species.

Indexing (document details)
Advisor: Melzer, Michael
Commitee: Cheng, Zhiqiang, Sipes, Brent
School: University of Hawai'i at Manoa
Department: Tropical Plant Pathology
School Location: United States -- Hawaii
Source: MAI 81/2(E), Masters Abstracts International
Subjects: Entomology, Agriculture, Plant sciences
Keywords: Coconut rhinoceros beetle, Haplotype, High-throughput sequencing, Pheromone, qPCR, Virus discovery
Publication Number: 13865466
ISBN: 9781085590433
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