Through the creation of a 2D and 3D model of human skeletal muscle, structural and functional data can be collected. The initiation mechanisms of Facioscapulohumeral muscular dystrophy’s (FSHD), a genetic dystrophy that causes increasing muscle degeneration, can be identified through the creation of a reproducible model. Here, we share how the details of various facets and properties of the 2D and 3D design affect a human skeletal muscle in vitro. The specifics of how various extracellular matrix play a role in 2D patterning of skeletal muscle is discussed alongside the intricacies of creating a hydrogel based 3D skeletal muscle bundle. While the models have not yet reached optimal design compared to other techniques, this design is close to reaching a state to produce collectible data.
|Commitee:||Botvinick, Elliot, Yokomori, Kyoko|
|School:||University of California, Irvine|
|Department:||Biomedical Engineering - M.S.|
|School Location:||United States -- California|
|Source:||MAI 81/2(E), Masters Abstracts International|
|Keywords:||FSHD, Geltrex, Human skeletal muscle|
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