This quantitative study was designed to investigate how interactions with mentors during a project affected a students’ motivation and interest in STEM disciplines and careers. This study utilized the STEM Semantics Survey to quantify student interest in science, the Career Interest Questionnaire to quantify student interest in STEM based careers, and the Mentorship Quality Experience Survey to quantify their perceived quality of the mentor. The STEM Semantics Survey, and Career Interest Questionnaire were both administered to all 76 students participating prior to and at the end of the four-week project. The Mentorship Quality Experience Survey was only administered to the 53 students who received a mentor. Findings suggest that female students’ interest in science were positively affected by their mentorship experience. Recommendations to teachers include the development of mentorship experiences in the classroom because of the positive impact it has on female students’ perception of science disciplines.