Self-efficacy has a profound impact on sport, with numerous studies over the past several years showing a significantly high positive correlation between self-efficacy and sport performance. This relationship has been demonstrated in a variety of sports, but has yet to be elucidated within the sport of rowing. The purpose of this study is to investigate whether self-efficacy predicts performance in collegiate rowers during a 2,000-meter rowing ergometer test (2k test). Twenty-one collegiate club rowers (14 men; 7 women) completed a demographic measure, the Primary Appraisal Secondary Appraisal measure (PASA) and the Rowing Efficacy Inventory–Individual (REI-I) prior to performing the 2k test, and then retook the PASA after. One male participant was forced to stop the 2k test briefly, and was therefore removed from analysis. Due to significant differences in 2k test performance between male participants (M = 440.85, SD = 19.38), and female participants (M = 519.80, SD = 32.12), t (19) = −6.91, p <.001, a linear regression test was run separately for each gender. This test showed that self-efficacy significantly predicted 2k test performance in male rowers, b = −.623, t (11) = −2.63, p = .023, and self-efficacy also explained a significant proportion of variance in 2k test performance, R² = .39, F (1,11) = 6.96, p = .023. Future studies should aim to examine how self-efficacy impacts on-water performance in rowers, as well as continue to understand differences in efficacy beliefs between genders.