The Nutritional Facts label is intended to make it easier for people to make better diet-related decisions, yet research has shown that the label is not generally understood. This thesis examines users’ comprehension of information on the Nutrition Facts label via an online survey. Results from a 2 (Line Weight: present or absent) × 2 (5-20 Rule: present or absent) × 2 (Color: present or absent) × 2 (Serving Size Definition: present or absent) analysis of variance (ANOVA) on overall accuracy indicated that including the definition Serving Size was beneficial to performance. The use of Line Weight, Color, or the 5-20 Rule did not improve participants’ accuracy in interpreting information on the Nutrition Facts label in most cases. Thus, it is recommended to include the definition of serving size on the label to help improve users’ understanding of that information on the label.