A new ethanol detailed kinetic model with 107 species and 1795 reactions was developed by using the reaction mechanism generator (RMG) and a thorough reaction path analysis. The mechanism model was extensively evaluated against measured ignition delay times, laminar flame speeds, and time-resolved species concentrations. The ignition delay experiments were conducted at pressures of 15, 20, and 30 bar, a temperature range of 850 to 1000 K, and equivalence ratios of 0.5, 1.0 and 2.0 using an optically accessible rapid compression machine (RCM). The effect of oxygen concentration on the ignition delay at a fixed equivalence ratio was also measured and studied using the new kinetic model. High speed camera was used to investigate the autoignition process and chemiluminescence emission at low to intermediate temperatures. Different combustion behaviors with respect to the chemiluminescence color and intensity were identified during the autoignition of ethanol mixture. The new combustion kinetic model predicts the measured data from this research and those available in the literature very well.