Dissertation/Thesis Abstract

An Investigation on How 8-Oxo-7,8-Dihydrogaunosine Affects RNase A, RNase T1, and XRN1 Reactivity with Oligonucleotides of RNA and RNA Structure Using the Theophylline Binding Aptamer as Model
by Kiggins, Courtney N., M.S., University of Colorado at Denver, 2019, 70; 13865629
Abstract (Summary)

8-Oxo-7,8-dihydroguanosine (8-oxoG) is a result of oxidative damage on the nucleobase Guanosine. 8-OxoG has been well characterized in DNA, but little is known of its function in RNA. The purpose of this study was to understand how 8-oxoG influences enzyme reactivity, RNA structure, and RNA function. First, endoribonucleases Ribonuclease A (RNase A) and Ribonuclease T1 (RNase T1) were studied to understand 8-oxoG recognition. It was found that 8-oxoG becomes a substrate for RNase A, while it was not recognized by RNase T1. The functional group on C8 causes an anti to syn flip which exposes new hydrogen bonding patterns similar to that of Uridine, which allowed RNase A reactivity. The conformational change, though, causes 8-oxoG to lose its ability to be a substrate for RNase T1 due to sterics and adverse hydrogen bonding induced by the exocyclic functional group at C8. The information gathered from the endoribonucleases was then applied to study how 8-oxoG begets structural and functional changes to a well-studied aptamer, the theophylline binding aptamer. 8-OxoG insertion caused a 100-fold increase in dissociation constant (Kd) for the theophylline binding aptamers modified at positions G25 and G26, while a modification at G11 prevented the aptamer from binding to theophylline. RNase A and T1 degradation data also yielded different degradation sites in the modified aptamers compared to the canonical, indicating a change in structure. Following, the reactivity of exoribonuclease XRN1 was studied in the presence of oligomers containing one to three 8-oxoG modifications due to XRN1’s role in oxidized mRNA surveillance. XRN1 was found to stall at 8-oxoG sites as a function of number of 8-oxoG present when more than one modification was introduced. The data combined helps provide insights into how 8-oxoG may affect RNA decay and surveillance mechanisms.

Indexing (document details)
Advisor: Resendiz, Marino J.E.
Commitee: Fisk, John D., Ren, Xiaojun, Wang, Haobin
School: University of Colorado at Denver
Department: Chemistry
School Location: United States -- Colorado
Source: MAI 58/06M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Chemistry, Biochemistry
Keywords: 8-OxoG, Microscale thermophoresis, RNase A, RNase T1, Theophylline binding aptamer, XRN1
Publication Number: 13865629
ISBN: 9781392275290
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