The isolation and detection of circulating tumor cells (CTCs) is becoming more important in the field of biomedicine as well as the diagnosis and treatment of cancer. Cancer metastasis is a set of occurrences where cells are shed from a cancerous site, then flow throughout the circulatory system and seed themselves throughout the body forming secondary tumors. Clinical treatment is limited by the lack of early symptoms of metastasis. This thesis presents the fabrication and preliminary test results of a magnetophoretic bioseparation chip capable of isolating and detecting CTCs from peripheral blood which can aid in early detection of metastases. This chip consists of two 25mm × 75mm × 1.0mm glass microscope slides which are bonded together by a piece of polyimide film to create a fluidic channel with a height of 200 μm. A blood sample is spiked with MCF7 breast cancer cells and mixed with superparamagnetic microparticles that are specifically coated with EpCAM antibodies to bind to such cells. The blood sample containing the MCF7 breast cancer cells is then introduced into the microfluidic chip where the magnetically labeled cancer cells are captured by a locally engineered magnetic field gradient while the non-target cells flow through the chip. After the isolation process the purity and recovery rate of the cancer cells are determined by flow cytometry. This microfluidic chip is unique in that it can operate at continuous flow rates magnitudes higher than most other CTC isolation devices. The combination of high throughput and simplicity of design make it possible for economic point of care use in clinical applications.
|Commitee:||Celik, Serdar, Schober, Joe|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 58/06M(E), Masters Abstracts International|
|Keywords:||Immunomagnetic detection, Liquid biopsy, MCF7, Magnetophoresis, Microelectromechanical systems|
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