Dissertation/Thesis Abstract

Bacteria Associated with Microbialites at Pisa Pond, Death Valley National Park, California
by Levish, Michelle, M.S., California State University, Long Beach, 2019, 112; 13424973
Abstract (Summary)

Pisa Pond, a saline pool in the Badwater salt pan, Death Valley, hosts microbialites with manganese oxyhydroxides. The aims of this study were to characterize bacteria from two depths within the microbialites via 16S rRNA gene sequencing and metagenomics, isolate and characterize Mn(II)-oxidizing bacteria physiologically and genomically, and to identify the manganese minerals present. Bacterial community composition and metagenomes were not statistically different between depths. However, differences in community structure and metabolic pathways were observed. Unlike other saline microbialites, Cyanobacteria were not abundant. A Photobacterium sp. which oxidized Mn(II) only in biofilms was isolated. Genomic analysis revealed the isolate had horizontally-acquired genes, including those for multicopper oxidase, that may be linked to its oxidation activity. The manganese minerals were poorly ordered and could not be identified. These data reveal a diverse community distinct from other microbialites that, along with environmental factors, contribute to the production of manganese oxyhydroxides.

Indexing (document details)
Advisor: Dillon, Jesse
Commitee: Berlemont, Renaud, Stevens, Lora
School: California State University, Long Beach
Department: Biological Sciences
School Location: United States -- California
Source: MAI 58/05M(E), Masters Abstracts International
Subjects: Microbiology
Keywords: Death valley, Manganese, Microbialites, Photobacterium
Publication Number: 13424973
ISBN: 978-1-392-15986-6
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