Mass spectrometry (MS) is an efficient analytical tool due to a number of advantages for providing high sensitivity, high-throughput analysis and less sample consumption. Since 1897, MS has continued to evolve and significant developments have occurred in the area of ionization techniques. Since 2010, inlet ionization techniques have shown the potential to compete in the areas of sensitivity and sample conservation with electrospray ionization (ESI) and matrix assisted laser desorption/ionization (MALDI). Matrix assisted inlet ionization (MAI) produces multiply charged ions within a mass spectrometer inlet without the need of high voltage, a laser or nebulizing gas. For MAI, sample preparation can be critical affecting the sensitivity, reproducibility and carry over. This dissertation presents new sample preparation techniques which have shown substantial improvement in sensitivity and reproducibility. The limit of detection can be achieved to less than 50 amol with the new sample preparation technique. Furthermore, this new sample introduction process enhance matrix assisted ionization (MAI) and solvent assisted ionization (SAI)’s ability to couple with Thin-layer chromatography.
|Advisor:||McEwen, Charles N.|
|Commitee:||Gupta, Pardeep, Pagnotti, Vincent S., Sidorenko, Alexander, Tomsho, John W.|
|School:||University of the Sciences in Philadelphia|
|School Location:||United States -- Pennsylvania|
|Source:||DAI-B 80/08(E), Dissertation Abstracts International|
|Subjects:||Chemistry, Analytical chemistry|
|Keywords:||Inlet ionization, MAI, Mass spectrometry, SAI, TLC|
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