Progress in systems neuroscience requires effective tools and techniques for probing neural circuits, and for analyzing the resulting data in ways that drive theoretical insight. This thesis consists of three parts, aimed broadly toward furthering the measurement and analysis of neural circuits. In the first part, we present methods for two-photon imaging of brain regions situated in deep fissures, enabling the use of cellular resolution optical tools for probing areas such as the medial prefrontal cortex (mPFC) and medial entorhinal cortex (MEC). We demonstrate recordings of population activity in the mPFC and grid cells in the MEC in behaving mice. In the second part, we present an optical approach for measuring dopaminergic input to the mPFC with high spatiotemporal resolution, which has not been feasible using traditional methods. We demonstrate recordings of mPFC dopamine signals in behaving mice, and present preliminary evidence for fine-scale heterogeneity across individual dopaminergic axons. In the third part, we present a new unsupervised learning algorithm for inferring underlying, nonlinear structure in neuronal population activity. We use this algorithm to characterize the geometric properties of hippocampal activity and their relationship to behavior. And, we propose a conceptual model explaining how neural coding and trial-to-trial variability both arise from movement along a low dimensional, nonlinear activity manifold, driven by internal cognitive processes.
|Advisor:||Tank, David W.|
|Commitee:||Brody, Carlos D., Fiete, Ila R., Pillow, Jonathan W., Wang, Samuel SH|
|School Location:||United States -- New Jersey|
|Source:||DAI-B 80/08(E), Dissertation Abstracts International|
|Keywords:||Dopamine, Grid cells, Hippocampus, Manifold, Prefrontal cortex, Two-photon microscopy|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be