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Dissertation/Thesis Abstract

The pursuit of membrane protein structures and the malarial aquaglyceroporin pfAQP
by Newby, Zachary Eric Robert, Ph.D., University of California, San Francisco, 2008, 208; 3339213
Abstract (Summary)

When I began my thesis work, structures of membrane proteins were few and far between. It was clear that the field was poised for a growth period and offered myriad opportunities for pursuing targets involved in important and interesting biology. It was also clear that there was a dire need to develop new methods and protocols to express, purify and crystallize membrane proteins. Described here are my efforts to contribute to this growth period of membrane protein structural biology. This includes establishing cell culture facilities and protocols for the production of milligram quantities of mammalian membrane proteins, the solving of the structure of the Malarial aquaglyceroporin PfAQP and a carefully designed expression screen that has resulted so far in the crystallization of four proteins. The structure of one of theses proteins, CcmG, has been solved.

Indexing (document details)
Advisor: Stroud, Robert M., Julius, David
Commitee: Edwards, Robert, Weissman, Jonathon
School: University of California, San Francisco
Department: Chemistry and Chemical Biology
School Location: United States -- California
Source: DAI-B 69/12, Dissertation Abstracts International
Subjects: Biochemistry, Biophysics
Keywords: Aquaglyceroporin, Crystallography, Expression, Membrane proteins, Purification, Reconstitution
Publication Number: 3339213
ISBN: 978-0-549-94964-0
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