Dissertation/Thesis Abstract

Hydroxyproline-O-glycosylation in Monocot Plants and Its Application in Cell Wall Engineering
by Verma, Neha, M.S.A., Arkansas State University, 2019, 68; 13805627
Abstract (Summary)

Switchgrass (Panicum virgatum) is a perennial C4 grass that has been most extensively studied as a dedicated bioenergy crop. However, efficient conversion of switchgrass biomass to biofuels has been hampered by biomass recalcitrance. Genetic modification of the plant cell wall represents a promising solution to overcoming this problem. The goal of this project is to leverage an innovative strategy, the hydroxyproline (Hyp)-O-glycosylation "code", for de novo design and engineering in switchgrass of novel designer biopolymers (DBPs) to facilitate cell wall reconstruction. The Hyp-O-glycosylation of the engineered DBPs was characterized. The results from this study show; embryogenic switchgrass calli were successfully generated, the engineered recombinant HypGP tagged EGFPs {(SP)32-EGFP and (SP4)18-EGFP} were Hyp-O-glycosylated in switchgrass and rice, in whole plants or cultured cells, the Hyp-O-glycosylation of the HypGP tag can be performed by monocot plants the same as in dicot plants and no phenotypic significant difference of transgenic plants was observed.

Indexing (document details)
Advisor: Hood, Elizabeth E., Xu, Jiangfeng
Commitee: Green, Steven, Phillips, Gregory
School: Arkansas State University
Department: Agriculture
School Location: United States -- Arkansas
Source: MAI 58/04M(E), Masters Abstracts International
Subjects: Molecular biology, Agriculture, Plant sciences
Keywords: (SP)32-EGFP and (SP4)18-EGFP, Cell wall engineering, Designer biopolymers, Hydroxyproline-o-glycosylation, Stable transformation of switchgrass callus, Transient gene expression
Publication Number: 13805627
ISBN: 978-0-438-93938-7
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