Background: In surgical procedures, biomaterials such as membranes are exposed to microbiota present within the oral cavity during manipulation. These include early colonizers of the streptococci species along with putative periodontal pathogens even after debridement. Furthermore, guided tissue regeneration membranes which harbor lower levels of microbiota exhibit greater attachment gain. Therefore, the selection of an adjunctive biomaterial that contains anti-microbial properties may enhance the regenerative outcome. The purpose of this study is to evaluate wound biomodification by assessing antimicrobial properties present within a human derived composite amnion-chorion membrane (ACM).
Methods and Materials: Membranes analyzed were the human derived ACM BioXclude® (Snoasis Medical) and the porcine derived collagen membrane Bio-Gide® (Geistlich). Blank paper discs served as a negative control along with paper discs containing tetracycline (TCN) at a bactericidal concentration of 62 μg/mL as a positive control. Aggregatibacter actinomycetemcomitans (A.a.), Streptococcus mutans (S.m.), and Streptococcus oralis (S.o.) were chosen for study.
The same number of colony forming units per milliliter (CFU/mL) for each bacterial species was inoculated on each membrane and control disc in triplicate for three separate trials. Samples were grown over brain heart infusion (BHI) agar medium under optimal conditions. Discs from each group were removed at 12 and 24 hours and sonicated to remove the bacteria off the membranes. A serial dilution was performed to quantify bacterial growth by counting the CFU/mL present. A Wilcoxon signed-rank test was performed to compare any differences between growths.
Results: Three separate in vitrof trials were conducted for a total of two-hundred sixteen data points to determine median microbial counts. The ACM inhibited growth at all time points, with all bacterial species, identical to the positive control TCN discs. The collagen membrane and negative paper controls did not inhibit growth of any of the bacterial species throughout the 24-hour study period.
Conclusions: This is the first study to demonstrate antimicrobial properties present within a regenerative membrane. ACM was proven to be as bactericidal as paper discs treated with TCN at a bactericidal concentration. The collagen membrane does not appear to have antimicrobial properties due to its support of the bacterial growth similar to the negative control discs. The findings from this study arm the practitioner with important information to enhance surgical outcomes.
|Commitee:||Lucas, Mark, Powell, Charles A., Schurr, Michael|
|School:||University of Colorado at Denver|
|Department:||Health Information Technology|
|School Location:||United States -- Colorado|
|Source:||MAI 58/02M(E), Masters Abstracts International|
|Keywords:||Amnion-chorion, Bactericidal, Basic science, Biomaterials, Membranes, Periodontics|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be