Dissertation/Thesis Abstract

In Vitro Analysis of Antimicrobial Activity Between an Amnion-chorion Membrane as Compared to a Collagen Membrane
by Ashraf, Haroon, M.S., University of Colorado at Denver, 2017, 68; 10275590
Abstract (Summary)

Background: In surgical procedures, biomaterials such as membranes are exposed to microbiota present within the oral cavity during manipulation. These include early colonizers of the streptococci species along with putative periodontal pathogens even after debridement. Furthermore, guided tissue regeneration membranes which harbor lower levels of microbiota exhibit greater attachment gain. Therefore, the selection of an adjunctive biomaterial that contains anti-microbial properties may enhance the regenerative outcome. The purpose of this study is to evaluate wound biomodification by assessing antimicrobial properties present within a human derived composite amnion-chorion membrane (ACM).

Methods and Materials: Membranes analyzed were the human derived ACM BioXclude® (Snoasis Medical) and the porcine derived collagen membrane Bio-Gide® (Geistlich). Blank paper discs served as a negative control along with paper discs containing tetracycline (TCN) at a bactericidal concentration of 62 μg/mL as a positive control. Aggregatibacter actinomycetemcomitans (A.a.), Streptococcus mutans (S.m.), and Streptococcus oralis (S.o.) were chosen for study.

The same number of colony forming units per milliliter (CFU/mL) for each bacterial species was inoculated on each membrane and control disc in triplicate for three separate trials. Samples were grown over brain heart infusion (BHI) agar medium under optimal conditions. Discs from each group were removed at 12 and 24 hours and sonicated to remove the bacteria off the membranes. A serial dilution was performed to quantify bacterial growth by counting the CFU/mL present. A Wilcoxon signed-rank test was performed to compare any differences between growths.

Results: Three separate in vitrof trials were conducted for a total of two-hundred sixteen data points to determine median microbial counts. The ACM inhibited growth at all time points, with all bacterial species, identical to the positive control TCN discs. The collagen membrane and negative paper controls did not inhibit growth of any of the bacterial species throughout the 24-hour study period.

Conclusions: This is the first study to demonstrate antimicrobial properties present within a regenerative membrane. ACM was proven to be as bactericidal as paper discs treated with TCN at a bactericidal concentration. The collagen membrane does not appear to have antimicrobial properties due to its support of the bacterial growth similar to the negative control discs. The findings from this study arm the practitioner with important information to enhance surgical outcomes.

Indexing (document details)
Advisor: Font, Kerri
Commitee: Lucas, Mark, Powell, Charles A., Schurr, Michael
School: University of Colorado at Denver
Department: Health Information Technology
School Location: United States -- Colorado
Source: MAI 58/02M(E), Masters Abstracts International
Subjects: Dentistry
Keywords: Amnion-chorion, Bactericidal, Basic science, Biomaterials, Membranes, Periodontics
Publication Number: 10275590
ISBN: 978-0-438-53309-7
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