Production, distribution, and disposal of pharmaceutical products, especially Endocrine disrupting chemicals (EDCs), have become a global issue. The presence of EDCs in the environment, even months and years after their application may result in disruption of the homeostatic system in various organisms. Although there is still insufficient evidence of the adverse effect of EDCs on humans at environmental levels, studies show that these chemicals have been suspected to increase the risk of various cancers, as well as infertility and other reproductive problems. The aim of this project is to study the scope of accumulation of three of the most commonly used beta blockers and their effect on regeneration on Brown planaria. Acute toxicity tests were conducted for each beta blocker in order to determine the LC50s. Calculated LC50s for acebutolol, metoprolol and propranolol were 778 ppm, 711 ppm, and 11.1 ppm, respectively. Planaria with similar size and mass were exposed to 1, 10 and 100ppb spiked solutions of acebutolol, metoprolol and propranolol for 2, 4, 5, 6, 7 and 14 days. The analysis was performed by LC-MS/MS to quantify the EDCs accumulation in Planaria. Results of this study showed that after 4 days of exposure to beta-blockers, the accumulation drastically decreased for all three beta blockers in all concentrations which suggests that Planaria might have an effective defense mechanism against this type of pharmaceuticals. The exposure water was tested after each exposure period and it was found that the concentration of beta blockers in the solution increases after the 4th day, suggesting that Planaria excretes the unmetabolized compound to the water. Additionally, Planaria were cut proximal to the head and the two fragments were placed in separate Petri dishes filled with 100 ppb spiked solution of beta-blockers and the quality of regeneration was documented from each fragment daily. Comparing the images taken from Planaria in the presence of the beta-blockers with control samples, no significant difference was observed after 2 weeks of regeneration by comparing the regeneration profile in Planaria placed in beta-blocker solution and control solution.
|Advisor:||Tucker, Kevin R.|
|Commitee:||Sumita, Mina, Wiediger, Susan|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 58/01M(E), Masters Abstracts International|
|Subjects:||Chemistry, Environmental science|
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