Dissertation/Thesis Abstract

The Effect of Repeated Cold Exposure on Insect Immunity
by Karnish, Candace, M.S., Southern Illinois University at Edwardsville, 2018, 60; 10844278
Abstract (Summary)

Most studies examining effects of extreme temperature on insect immunity have focused on negative impacts of high-temperature exposure. Although largely unexplored, a recent study suggests that repeated exposure to freezing temperature may enhance insects’ ability to survive microbial challenge. To determine if repeated low-temperature exposure impacts innate immunity of the goldenrod gall fly, Eurosta solidaginis, we counted hemocyte number, measured phenoloxidase activity and assessed antibacterial activity, and I examined up-regulation of immune-related genes in winter-acclimated larvae 24 h or 96 h after they were subjected to either zero (control), five, or ten diurnal exposures to -18°C. To assess if time spent frozen, rather than freezing and thawing, influenced immunity, we also counted examined immune factors in larvae exposed to a single, six-day constant freeze. Repeated freezing negatively impacts hemocyte number, antimicrobial activity against Gram-negative bacteria, and had varied effects on phenoloxidase activity in the goldenrod gall fly. I suggest the effects of repeated cold exposure on insect immunity are governed by energy trade-offs and are not likely to mimic the effects of a single freeze.

Indexing (document details)
Advisor: Williams, Jason, McCracken, Vance
Commitee: DiSalvo, Susanne, McCracken, Vance, Williams, Jason
School: Southern Illinois University at Edwardsville
Department: Biological Sciences
School Location: United States -- Illinois
Source: MAI 58/01M(E), Masters Abstracts International
Subjects: Entomology, Microbiology, Immunology
Publication Number: 10844278
ISBN: 978-0-438-37899-5
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