Ovulation is regulated by feedback mechanisms that include systems of astrocyte-neuronal interactions responsive to estradiol and progesterone in the rat. Estradiol induces progesterone receptors (PGRs) in rostral periventricular region of the third ventricle (RP3V) kisspeptin neurons, and positive feedback estradiol concentrations induce neuroprogesterone (neuroP) synthesis in hypothalamic astrocytes that signal to PGRs expressed in kisspeptin neurons to trigger the LH surge. I tested the hypothesis that neuroP-PGR signals through Src family kinase (Src) to trigger the LH surge. PGR and Src are co-expressed in RP3V neurons and their colocalization and immunopositive cells are upregulated with estradiol treatment. RP3V infusions of Src inhibitor (PP2) attenuated the LH surge in 50µg EB primed ovariectomized/adrenalectomized (ovx/adx) rats. In 2µg EB primed animals, infusions of Src activator and PGR triggered the LH surge. These results support my hypothesis that neuroprogesterone signals through RP3V PGR-Src complexes in kisspeptin neurons to induce the LH surge.
|Commitee:||Micevych, Paul, Young, Kelly|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 58/01M(E), Masters Abstracts International|
|Keywords:||Estradiol, Kisspeptin, Luteinizing hormone surge, Neuroprogesterone, Ovulation, RP3V|
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