Dissertation/Thesis Abstract

Investigation of Antimicrobial Peptide Genes in Maize ( Zea mays) Inbred Lines Resistant to Lepidoptera Larvae Feeding and Fungus Infection
by Noonan, Joseph Ali, M.S., Mississippi State University, 2018, 174; 10841223
Abstract (Summary)

Present throughout all classes of life, antimicrobial peptides (AMPs) confer defense against bacteria, viruses, fungi, and insects. Identifying maize AMPs would provide breeders with a new defense resource. Here, the investigation of maize AMPs is reported. The distribution of AMPs within a panel of ten Mississippi maize inbred lines with varying resistance to Lepidoptera larvae feeding and Aspergillus infection is explored to characterize their observed resistances. Homology data-mining with two comprehensive AMP databases revealed 88 unique maize AMP protein sequences across 81 genes in the MaizeGDB B73 genome assembly. AMP-related polymorphic sites were identified using genomic primers. Analyses with qRT-PCR revealed 8 differentially expressed maize AMP genes. Computational 3D models of AMPs are limited, and models of these eight maize AMP genes were predicted. Two-dimensional electrophoresis gels were used to contrast protein profiles of inbred lines with varying resistance to identify regions related to AMPs and other defense-related protein.

Indexing (document details)
Advisor: Shan, Xueyan
Commitee: DuBien, Janice, Ma, Din-Pow, Williams, William P.
School: Mississippi State University
Department: Biochemistry and Molecular Biology
School Location: United States -- Mississippi
Source: MAI 58/01M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Biochemistry
Keywords: Antimicrobial peptides
Publication Number: 10841223
ISBN: 9780438313712
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