Dissertation/Thesis Abstract

Isolation and Characterization of a Prolactin-Regulating Factor (PRF) from a Mouse Pituitary Intermediate Lobe Cell Line
by Hnasko, Robert, Ph.D., University of Cincinnati, 2000, 189; 10857377
Abstract (Summary)

Prolactin (PRL) is regulated by inhibitory and stimulatory factors from the hypothalamus and pituitary. Dopamine is the primary PRL-inhibitory factor, but acute elevations in PRL require stimulation by a PRL-releasing factor (PRF). PRF from the rat pituitary rapidly stimulated PRL release and localized to the intermediate lobe (IL). Partial purification of multiple PRF species from IL tumors differed in size and chromatographic properties. However, the primary structure of PRF has not been resolved. Our hypothesis was: a subpopulation of IL cells secretes a novel peptide that functions as a PRF. Transgenic POMC-Tag mice that develop IL tumors were used to investigate the following objectives: 1) determine if POMC-Tag mice with IL tumors develop hyperprolactinemia, 2) establish and characterize an IL cell line the produces PRF, 3) determine the biochemical properties of PRF, 4) purify PRF and determine its structure. Hyperprolactinemia was not observed in POMC-Tag mice from 20 to 120 days. Cells from two IL tumors were cloned into mIL cell lines that differed in cellular characteristics. As assessed by RT-PCR the mIL39, but not mIL5, cells expressed POMC and D2R gene products, characteristic of IL melanotrophs. The cell lineage of mIL5 cells remains undefined. Only the mIL5 cells, co-cultured with GH3/luc cells, stimulated PRL gene expression and release. PRF from mIL5 was secreted and bound heparin. Two heparin-binding proteins, FGF2 and HB-EGF, identified in mIL5 cells were potent PRL inducers. However, neither significantly contributed to the PRF activity from mIL5 as judged by Western blotting, heparin-affinity and immunoneutralization.

Purification of PRF from mIL5 by heparin-affinity chromatography indicated multiple species that differed in chromatographic properties. Sequential chromatography was used to isolate PRF from cell extract and sequencing identified a peptide identical to an internal sequence of heparin-interacting protein (HIP/L29). The expression of HIP protein was ubiquitous and two HIP antibodies were ineffective in the attenuation of PRF activity from mIL5 cells.

Isolated PRF from conditioned media was subjected to mass spectrometry that revealed a mass of 14,968 Da. N-terminal sequence analysis failed to resolve the primary structure of PRF.

Indexing (document details)
Advisor: Ben-Johnson, Nira
School: University of Cincinnati
Department: Medicine
School Location: United States -- Ohio
Source: DAI-B 79/10(E), Dissertation Abstracts International
Subjects: Medicine
Keywords: Cell liens, Heparin-binding growth factors, Pituitary, Prolactin
Publication Number: 10857377
ISBN: 978-0-438-03502-7
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