Dissertation/Thesis Abstract

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Highly Multiplex Imaging Using the Primer Exchange Reaction Method, and the Study of Potential Hydrogels for the Application of Expansion Microscopy
by Lin, Millicent, M.S., Tufts University, 2018, 105; 10810763
Abstract (Summary)

We have proposed a method using the primer exchange reaction (PER) in which allows synthesis of single stranded DNA using a catalytic hairpin to achieve highly multiplex resolution imaging. PER cascades are an ideal candidate in amplifying signals with in vitro extended probes in a programmable fashion. In parallel, we have studied the mechanical properties and contributing factors of different hydrogel composition in the context of expansion microscopy (ExM). ExM enables imaging of cells and tissues with nanoscale precision.

Indexing (document details)
Advisor: Wang, Yu, Church, George
Commitee: Cronin-Golomb, Mark, Jiang, Xiaocheng, Wang, Yu
School: Tufts University
Department: Biomedical Engineering
School Location: United States -- Massachusetts
Source: MAI 57/06M(E), Masters Abstracts International
Subjects: Biomedical engineering
Publication Number: 10810763
ISBN: 9780438020344
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