Dissertation/Thesis Abstract

Statistical Analysis of cDNA Microarrays: A Systematic Approach to Identify MTF-1 Mediated Gene Expression Profile in Response to Cadmium
by Yang, Yi, M.S., University of Cincinnati, 2003, 69; 10857152
Abstract (Summary)

In this particular study, we compared the global gene expression values from two nearly identical cell lines except the presence or absence of the transcription factor MTF-1. The goal is to identify MTF-1 mediated gene expressions after cadmium treatment. The initial data pre-processing steps include data transformation and normalization. The normalized data were then filtered and the remaining data were clustered using five clustering algorithms. The clustering results were evaluated on their sensitivity in detecting patterns and on their power of prediction. Our results suggest that the model based clustering offered a better approach than the hierarchical clustering. Furthermore, the Bayesian infinite mixture model based clustering showed the best gene expression patterns that were relevant to biological findings. Two groups of genes were identified to be either up- or down-regulated through MTF-1 in response to Cadmium. Such information will provide further insights in characterizing the function of this transcription factor.

Indexing (document details)
Advisor: Medvedovic, Mario
School: University of Cincinnati
Department: Environmental Health Sciences
School Location: United States -- Ohio
Source: MAI 57/06M(E), Masters Abstracts International
Subjects: Environmental science
Keywords: Cadmium, Clustering, Metal-response element-binding transcription factor-1, Microarrays
Publication Number: 10857152
ISBN: 978-0-438-02272-0
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