Helicobacter pylori is a bacterium that can cause chronic gastritis and gastrointestinal cancers in some infected people due to the overexpression of inflammatory cytokines and oxidative stress resulting from accumulation of reactive oxygen species. Verbesina negrensis is a plant that is routinely used in the treatment of gastric complaints. The purpose of this research is to isolate pure bioactive compounds from V. negrensis and evaluate the antimicrobial properties of the isolates against H. pylori. Additionally, we will determine the toxicity of the plant extracts on gastric epithelial cells (NCI-N87) using the MTT assay. We hypothesized that V. negrensis pure compounds would have anti-Helicobacter properties and low cytotoxicity to the epithelial cells. To test this hypothesis pure compounds were isolated from the V. negrensis ethanolic crude extract using silica gel and bioassay guided chromatography. Two pure compounds were isolated AG2 and AQ2B with MICs of 16 µg/mL and 128 µg/mL respectively. AQ2B was cytotoxic to NCI-N87 cells at MIC 128 µg/mL and sub-MIC values of 64, 32 and 16 µg/mL.
|Advisor:||McCracken, Vance J., Nieto, Marcelo|
|Commitee:||DiSalvo, Susanne, Kohn, Luci, McCracken, Vance J., Nieto, Marcelo|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 57/06M(E), Masters Abstracts International|
|Keywords:||Antimicrobial, Cytotoxicity, Helicobacter pylori, Verbesina negrensis|
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