One of the hallmarks of cancer is uncontrolled cell division. Our goal is to find and characterize novel genes that are important for cellular division. Characterizing novel cell cycle genes is an important step in gaining insight into mechanisms that regulate the cell cycle. Rb/E2F is a complex that controls the expression of genes that regulate the cell cycle. Previous research that utilized Affymetrix microarrays identified a potential novel E2F target. The sequence that was detected was an EST that corresponded to the mouse predicted protein D4Ertd421e. This sequence is 76% identical to a human protein, Cdca8. Cdca8, or cell division cycle associated 8, was given this name because its expression, based on EST frequencies, correlated with other cell cycle genes. cdca8 is regulated in a cell cycle-dependent manner, characteristic of Rb/E2F targets. cdca8 is also repressed in a p53-dependent manner, but basal levels of cdca8 are not solely controlled by p53. This suggests there are multiple pathways that control the expression of cdca8. The expression of cdca8 is also over represented in cancerous tissues. Two splice variants of cdca8 were identified, but they appear to have the same protein product and function the same in cells. Localization studies show a dynamiclocalization pattern for Cdca8, implicating it as a possible component of cellular division. These studies have provided further insight into the mechanisms of cellular division. The understanding of the molecular basis of the cell cycle is required for greater knowledge of cancer formation, prevention, and treatment.
|School:||The University of Toledo|
|School Location:||United States -- Ohio|
|Source:||MAI 57/05M(E), Masters Abstracts International|
|Keywords:||Cell cycle, Chromosomal passenger complex, P53, Rb|
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