Dissertation/Thesis Abstract

Ecology of Campylobacter Colonization in Poultry: Role of Maternal Antibodies in Protection and Sources of Flock Infection
by Orhan, Sahin, Ph.D., The Ohio State University, 2003, 190; 10834920
Abstract (Summary)

Although poultry are considered a major reservoir for Campylobacter, ecology of this human pathogen in chicken flocks is poorly understood, hampering the design of effective intervention strategies. First, the prevalence, antigenic specificity, and bactericidal activity of poultry Campylobacter maternal antibodies (MAB) were investigated. High levels of specific antibodies were detected in egg yolks, sera of broiler breeders, and young broiler chicks, as maternally-derived. MAB was against multiple outer membrane components of Campylobacter, and active in antibody-dependent complement-mediated killing of C. jejuni. Second, the effect of Campylobacter MAB on the colonization of young chickens was investigated using challenge studies. Laboratory inoculation of commercial broilers showed that onset of colonization occurred much sooner in birds challenged at the age of 21-days (which were negative for Campylobacter antibody) than it did in birds inoculated at 3-days of age (which were positive for Campylobacter MAB). Challenge experiments using specific pathogen-free (SPF) chickens demonstrated that significant decreases in the percentage of colonized chickens were observed in 3-day old SPF chicks with Campylobacter MAB as compared with 3-day old chicks without Campylobacter-specific MAB. These results indicated that Campylobacter MAB plays a partial role in protecting young chickens against colonization. Lastly, we investigated the ability of Campylobacter to penetrate eggshells, and its prevalence and survival within eggs. Campylobacter was either unable to penetrate the eggshell or did not survive up to 48 h inside incubating eggs. C. jejuni survived for up to 14 days in eggs stored at 18 °C following injection into the egg yolk; however, viability was dramatically reduced in the albumen or the air sac. Although Campylobacter was detected in fresh eggs obtained from an SPF flock colonized by C. jejuni, storage of eggs for 7 days at 18 °C resulted in the lack of detection. No Campylobacter was detected from 500 eggs laid by Campylobacter -colonized commercial breeders, and 1,000 eggs from a commercial hatchery, suggesting that vertical transmission of Campylobacter through the egg is unlikely.

Indexing (document details)
Advisor: Zhang, Qijing
School: The Ohio State University
Department: Veterinary Preventive Medicine
School Location: United States -- Ohio
Source: DAI-B 79/09(E), Dissertation Abstracts International
Subjects: Veterinary services
Keywords: Campylobacter, Chickens, Colonization, Maternal antibodies, Transmission
Publication Number: 10834920
ISBN: 9780355947007
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