The aim of this study was to investigate the effects of a low-cost minimal medium, developed by the University of Louisiana at Lafayette Bioprocessing Lab, on the cultivation of Lipomyces starkeyi NRRL Y-11557 under repeated feed-batch and continuous fermentation strategies, as well as the impact of lignocellulosic acid hydrolysis byproducts on the cocultivation of L. starkeyi with Acinetobacter baylyi. The highest cell and lipid concentrations were obtained under repeated feed-batch strategy. Continuous cultivation with dilution rate of 0.06h-1 presented the highest cell (0.401 g/g) and lipid yields (0.177g/g). Furthermore, the highest cell and lipid productivities were also obtained at the 0.06h-1 dilution rate under continuous cultivation. Exopolysaccharide production was observed when L. starkeyi was cultivated using a 90g/L glucose minimal media under the repeated feed-batch fermentation. The produced exopolysaccharide is likely composed of 4-5 repeating sugar units, incorporating mannose and galactose. A. baylyi was unable to grow on the developed minimal media, but cocultivation on minimal media with inhbitors (PHB, furfural, HMF, vanillin, and syringaldehyde) at various concentrations (0.1, 0.25 and 0.5g/L) yields comparable final cell and lipid production to media without inhibitors.
|Commitee:||Chistoserdov, Andrei, Revellame, Emmanuel, Xu, Wu|
|School:||University of Louisiana at Lafayette|
|School Location:||United States -- Louisiana|
|Source:||MAI 57/05M(E), Masters Abstracts International|
|Subjects:||Alternative Energy, Microbiology, Chemical engineering|
|Keywords:||Lipids, Microbial, Starkeyi|
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