An antisense oligodeoxynucleotide (ODN) is a piece of DNA that is complementary to the mRNA. This ODN will bind to the mRNA and prevent protein from being produced. This thesis describes the development and testing for efficacy of an antisense ODN to Sry, the testis determining factor. Cells were transfected with both a Sry expression vector and either the Sry antisense, sense or scrambled ODN. Western blots were run on the protein isolated from the cells. The amount of protein was decreased by 50% in the cells treated with the antisense Sry ODN when compared to the cells treated with the sense and scrambled ODNs. This implies that the antisense ODN did bind to the Sry mRNA and prevented the protein from being produced. This antisense Sry ODN was successful in decreasing the amount of Sry protein produced in the cells and this ODN could be used in future experiments.
|School:||The University of Akron|
|School Location:||United States -- Ohio|
|Source:||MAI 57/05M(E), Masters Abstracts International|
|Keywords:||Antisense, Spontaneous Hypertensive Rat, Sry1, oligodeoxynucleotide, sex determining region Y|
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