INTRODUCTION: Although recent studies have found that the lower respiratory tract microbiome was different in asthmatics compared to non-asthmatics in adults, the microbiome of asthmatic people with and without asthma exacerbation has not been well investigated.
OBJECTIVES: The primary objective of the study was to compare the abundance and diversity of the respiratory tract microbiome in children with and without asthma exacerbation.
METHODS: This was a cross sectional study where asthmatic children from 5-18 years old were recruited at the emergency department at LeBonheur Children’s Hospital, Memphis, TN. Recruited children were further grouped by asthma exacerbation level and asthma severity level. Induced sputum samples and nasopharyngeal swabs were collected to analyze the microbiome in the two sources with Illumina based sequencing. The primary outcome to measure was the microbiome abundance and diversity of specific taxa in the two sites with or without current asthma exacerbation. The secondary outcome to measure was the microbiome abundance and diversity of specific taxa in the two sites with various levels of asthma severity. The additional outcome to measure was the specific microbial taxa in the two sites associated with an increased or decreased risk of asthma exacerbation or severity.
RESULTS: 51 children from 5-10 years old were recruited. The relative abundance of 9 taxa (e.g. Moraxella) were significantly higher in nasopharyngeal samples, and the relative abundance of 26 taxa (e.g. Streptococcus) were significantly higher in induced sputum samples. No significant difference in abundance and diversity was found with various asthma exacerbation and severity levels in either of the two sites. No specific taxon was significantly associated with an increased or a decreased risk of asthma exacerbation or severity.
CONCLUSION: Microbiome composition differed significantly in induced sputum samples compared to nasopharyngeal samples. No significant change of microbial abundance and diversity in the two sites was associated with asthma exacerbation or severity. No specific taxon in the two sites was associated with an increased or a decreased risk of asthma exacerbation or severity. Larger, comparative studies need to be performed to discover subtle difference in future.
|Advisor:||Pettigrew, Melinda M.|
|Department:||School of Public Health|
|School Location:||United States -- Connecticut|
|Source:||MAI 57/05M(E), Masters Abstracts International|
|Keywords:||Asthma, Asthma exacerbation, Asthma severity, Microbiome, Pediatric asthmatics|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be