This study presents the numerical simulation and optimization of a dielectrophoretic bio-separation chip for isolating bioparticles such as circulating tumor cells (CTCs), or different strands of E.coli bacteria. The chip consists of ten pairs of slanted electrodes placed with an angle of 10° with respect to the direction of the flow on the top and bottom walls of the channel. The spatially non-uniform electric field produced by the electrodes applies a repulsive force on the particles that are flowing through the channel. The repulsive forces applied by the top and bottom electrodes are balanced and the particles stay at the centerline throughout the channel. The combination of forces in the x and z-directions deflects the particles depending on their size, and guides them towards different outlets. Numerical simulation of the particle-fluid transport was performed using the open-source software OpenFOAM, and the deflection of the particles within the microfluidic channel was predicted. The present computational domain considers the dominant forces such as the dielectrophoretic and hydrodynamic forces as well as their effects on the design and operating parameters. The results show that this device is capable of separating various cells with different sizes from their native environment. Furthermore, a parametric study was performed to investigate the effect of voltage, flow rate, number of electrodes, cell size, and channel height on the separation process and to improve the performance of the device.
|Commitee:||Molki, Majid, Shavezipour, Mohammad|
|School:||Southern Illinois University at Edwardsville|
|Department:||Mechanical and Industrial Engineering|
|School Location:||United States -- Illinois|
|Source:||MAI 57/04M(E), Masters Abstracts International|
|Subjects:||Engineering, Mechanical engineering|
|Keywords:||Bio-separation, Dielectrophoresis, Microfluidics, Slanted electrodes|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be