Dissertation/Thesis Abstract

The Neonatal Response to Gastric Helicobacter Using an H. felis Mouse Model of Infection
by Hoppenrath, Jean Marie, M.S., Southern Illinois University at Edwardsville, 2017, 42; 10680688
Abstract (Summary)

Infection with gastric Helicobacter can result in several gastric diseases, ranging from gastritis and ulcers to mucosa-associated lymphoid tissue (MALT) lymphoma and adenocarcinoma. Much of the Helicobacter -associated pathology is not a direct result of the infection, but rather from the resulting damage to an animal from its own immune response to the infection; this concept is also known as immunopathology. Previous studies using a Helicobacter felis-infected mouse model comparing mice infected as neonates or as adults showed that infected neonates have a delayed Th1/Th17 immune response. This delay may be attributed to the immature immune system in neonates.

The current study was undertaken to identify the period when infected neonates begin to exhibit a robust immune response. Previous studies that have demonstrated a lack of adaptive immune response in infected neonatal mice were based on 8-week trials. This study focuses on 16-, 26-, and 52-week infections. The anti-Helicobacter response was evaluated using serology.

For the 16-week trials, serology showed a four-fold decrease in the H. felis-specific antibody response in mice treated as neonates compared to mice treated as adults. Mice infected with H. felis as neonates had a significantly lower fecal secretory IgA response than mice infected with H. felis as adults. The serology for the 26-week trials showed a drastic increase in IgG production by mice infected with H. felis as neonates that is comparable to levels produced by adult H. felis-treated mice. This increase in systemic IgG denotes a stronger response at this particular time point. The serology of the 52-week trial was comparable to the results of the 26-week trial indicating a maintenance of a more robust response to infection. Mice reach immunologic maturity between weeks 6 and 8 weeks (11). Thus, our results show that even at 16-weeks of infection, well past immunologic maturity in mice, those treated as neonates have not exhibited an adult-like response.

Indexing (document details)
Advisor: McCracken, Vance J.
Commitee: Fowler, Thomas J., Jennings, Dave
School: Southern Illinois University at Edwardsville
Department: Biological Sciences
School Location: United States -- Illinois
Source: MAI 57/02M(E), Masters Abstracts International
Subjects: Molecular biology, Immunology
Keywords: Gastric, Helicobacter, Immune response, Neonatal
Publication Number: 10680688
ISBN: 978-0-355-57084-7
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