Infection with gastric Helicobacter can result in several gastric diseases, ranging from gastritis and ulcers to mucosa-associated lymphoid tissue (MALT) lymphoma and adenocarcinoma. Much of the Helicobacter -associated pathology is not a direct result of the infection, but rather from the resulting damage to an animal from its own immune response to the infection; this concept is also known as immunopathology. Previous studies using a Helicobacter felis-infected mouse model comparing mice infected as neonates or as adults showed that infected neonates have a delayed Th1/Th17 immune response. This delay may be attributed to the immature immune system in neonates.
The current study was undertaken to identify the period when infected neonates begin to exhibit a robust immune response. Previous studies that have demonstrated a lack of adaptive immune response in infected neonatal mice were based on 8-week trials. This study focuses on 16-, 26-, and 52-week infections. The anti-Helicobacter response was evaluated using serology.
For the 16-week trials, serology showed a four-fold decrease in the H. felis-specific antibody response in mice treated as neonates compared to mice treated as adults. Mice infected with H. felis as neonates had a significantly lower fecal secretory IgA response than mice infected with H. felis as adults. The serology for the 26-week trials showed a drastic increase in IgG production by mice infected with H. felis as neonates that is comparable to levels produced by adult H. felis-treated mice. This increase in systemic IgG denotes a stronger response at this particular time point. The serology of the 52-week trial was comparable to the results of the 26-week trial indicating a maintenance of a more robust response to infection. Mice reach immunologic maturity between weeks 6 and 8 weeks (11). Thus, our results show that even at 16-weeks of infection, well past immunologic maturity in mice, those treated as neonates have not exhibited an adult-like response.
|Advisor:||McCracken, Vance J.|
|Commitee:||Fowler, Thomas J., Jennings, Dave|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 57/02M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Immunology|
|Keywords:||Gastric, Helicobacter, Immune response, Neonatal|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be