Dissertation/Thesis Abstract

Surface Modification of Self-Assembled Graphene Oxide for Cell Culture Studies
by Swain, John E., III, M.S., North Carolina Agricultural and Technical State University, 2017, 75; 10620037
Abstract (Summary)

Thin films show great promise for biological applications, from in situ monitoring to pharmaceutical testing. In this study, a graphene oxide (GO) thin film is prepared with the aim to further functionalize the film for pharmaceutical toxicity screening applications. GO was selected due to its capability to be reduced into an optically transparent and electrically conductive thin film. In addition, GO is derived from carbon, a widely abundant element, in contrast to many other thin films that rely on resource-limited precious metals. Special care was taken to select GO and GO film synthesis methods that minimize the amount of organic-based solvents, maintain reactions at atmospheric pressure and moderate temperatures, and are scalable for manufacturing. Chemical oxidation of graphite flakes was carried out via a modified Hummer’s Method with a pre-oxidation step. The resulting GO flakes were self-assembled using commercially available 4-sulfocalix[4]arene. Analytical characterizations (e.g., elemental analysis, XRD, FTIR, Raman, SEM, AFM) were performed to evaluate the success of graphite oxidation and formation of the self-assembled thin film. In order to gain a better understanding of the interactions between GO and sulfocalix (SCX), equilibrium conformations of the SCX molecule and truncated GO were calculated using Spartan’16 Parallels. This study demonstrates that the interaction between the GO and the SCX molecule to create a self-assembled thin film is the result of π-π stacking, as hypothesized by Sundramoorthy et al. (2015). The self-assembled GO film was successfully deposited on a polyethylene terephthalate (PET) substrate and functionalized with 3-aminopropyl triethoxysilane (APTES), which renders the film capable of further functionalization with proteins for yielding a three-dimensional cell culture or co-culture platform for different applications.

Indexing (document details)
Advisor: Kuila, Debasish
Commitee: Aravamudhan, Shyam, Zhang, Lifeng
School: North Carolina Agricultural and Technical State University
Department: Nanoengineering
School Location: United States -- North Carolina
Source: MAI 57/01M(E), Masters Abstracts International
Subjects: Nanoscience, Nanotechnology
Keywords: 4-sulfocalix[4]arene, Cell culture, Functionalization, Graphene oxide, Self-assembly, Thin film
Publication Number: 10620037
ISBN: 978-0-355-30111-3
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