Dissertation/Thesis Abstract

Genetics of Resistance to Fusarium Wilt in Strawberry
by Pincot, Dominique Denise Anne, M.S., University of California, Davis, 2017, 87; 10624460
Abstract (Summary)

Fusarium wilt, a devastating disease caused by the soil-borne pathogen Fusarium oxysporum f. sp. fragariae, causes economic losses in strawberry (Fragaria × ananassa) worldwide. This disease was first identified in California in 2006 in fields where pre-plant fumigation with methyl bromide was no longer used. Fusarium wilt has since become a serious threat to production in California, which supplies a majority of strawberries produced in the US. A panel of 566 historically and commercially important F. × ananassa germplasm accessions were phenotyped for resistance in 2016 and 2017 by artificially inoculating field-grown plants with spores of a virulent isolate of F. oxysporum f.sp. fragariae found in California (AMP132) and were genotyped with 38,506 single-nucleotide polymorphisms (SNPs) using the iStraw35 Affymetrix Axiom array. In both years, Fusarium wilt resistance had high repeatability (0.983 and 0.896 in 2016 and 2017 respectively). Genome-wide association mapping using a diploid Fragaria vesca reference genome (v4.0) identified statistically significant (-log10(p-value) > 9) SNPs occurring in a 3 Mb region on chromosome 2 in both years, with these SNPs in linkage disequilibrium with resistance phenotypes. Genetic mapping in two self-pollinated mapping populations confirmed Mendelian segregation of a single dominant resistance gene. This R-gene, FoR2U-1, traces to early cultivars, predates the appearance of the disease in California by decades, and appears to provide strong resistance to the F. oxysporum isolate AMP132.

Indexing (document details)
Advisor: Knapp, Steven J.
Commitee: Brummer, Edward C., Gordon, Thomas R.
School: University of California, Davis
Department: Horticulture and Agronomy
School Location: United States -- California
Source: MAI 57/01M(E), Masters Abstracts International
Subjects: Horticulture, Plant sciences, Plant Pathology
Keywords: Fusarium, Oxysporum, Resistance, Strawberry
Publication Number: 10624460
ISBN: 978-0-355-45193-1
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