Dissertation/Thesis Abstract

Phospholipase A2 Enzymes: A Multidisciplinary Approach to Studying the Mechanisms of Activation and Inhibition
by Vasquez, Alexis Marie, M.S., University of California, San Diego, 2017, 86; 10607022
Abstract (Summary)

Phospholipase A2 is a superfamily of enzymes that play a major role in cellular homeostasis and disease development. This class of enzymes acts on a diverse set of phospholipids substrates, often releasing products that have a role in inflammatory pathways. Because phospholipases A2 play such a significant role in disease progression, understanding their mechanisms of action is vital in order to develop inhibitors for therapeutic interventions. In this thesis, the pathways of activation and inhibition are studied to learn about phospholipase’s structural dynamics and kinetic activity.

A computational study grounded in hydrogen deuterium exchange data reveals the binding mode of lipoprotein-associated phospholipase A2. Docking and molecular dynamic simulations were carried out using several substrates and a potent and selective inhibitor developed by GlaxoSmithKline. These results reveal a binding mode of lipoprotein-associated phospholipase A2 and how the enzyme is regulated by membrane allosterism. Membrane association causes a conformational change to occur, making it easier for substrates and inhibitors to enter the binding pocket. As ligands bind, lipoprotein-associated phospholipase A2 adjusts the binding pocket to fit around the ligand, ensuring a hydrophobic pocket forms so that catalytic hydrolysis may take place.

Activity assays were performed on calcium-independent phospholipase A 2 to observe enzyme regulation by ATP binding. This short study confirms that this enzyme has preferential cleavage toward certain head groups of substrates. It also demonstrates that concentrations of ATP up to 10µM activate calcium-independent phospholipase A2 when being analyzed using a high throughput LC-MS assay.

Finally, the hydrolytic activity of cytosolic phospholipase A2, calcium-independent phospholipase A2, and secreted phospholipase A2 toward phosphatidylinositols and phosphatidylinositol 4,5-bisphosphates was looked at. These preliminary studies reveal all three enzymes do not hydrolyze phosphatidylinositol 4,5-bisphosphates. Surprisingly, all thee enzymes act on phosphatidylinositols, with preferential cleavage of species that contained 16 and 18 carbon length chains.

Indexing (document details)
Advisor: Dennis, Edward A.
Commitee: McCammon, Andrew J., Muller, Ulrich
School: University of California, San Diego
Department: Chemistry
School Location: United States -- California
Source: MAI 57/01M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Chemistry, Biochemistry
Keywords: ATP, Activation, Computational, Inhibitor, PIP2, Phospholipase A2
Publication Number: 10607022
ISBN: 978-0-355-33316-9
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