Cultivation of adherently growing cells inside three dimensional scaffolds gains more importance as speciﬁc demands from medicine and industry arise. A huge challenge herein lies in nutrient supply all over the scaffold’s volume. This work presents a perfusion bioreactor device for cell cultivation inside porous aluminium oxide ceramics. The bioreactor supports vital growth of CHO-K1, A549, MDCK and human primary ﬁbroblasts on and in ceramic cylinders of 5 mm height and 10 mm diameter with perfusion velocities from 0.33-0.66 ml min-1.cm-2. Proliferation was assured by a metabolic assay based on resazurin reduction and carbon content analysis. Cell distribution and vitality was assessed by staining cross sections of the ceramic. Fibrous structures were observed inside ceramic scaffolds for perfusion cultivation of CHO-K1 and human primary ﬁbroblasts for several weeks by evaluation of scanning electron microscopy pictures. This clearly supports the hypothesis of micro milieu formation inside porous ceramics and consequently abundance of ECM-proteins. Perfusion therefore enables three-dimensional cell cultivation inside porous alumina ceramics.
|School:||Technische Universitaet Berlin (Germany)|
|Source:||DAI-C 81/1(E), Dissertation Abstracts International|
|Subjects:||Biomedical engineering, Materials science|
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