In light of their escalating exposure to possible environmental toxicants, there are many biological systems that need to be evaluated in a resource and time efficient manner. Understanding how toxicants behave in relation to their physicochemical properties and within complex biological media is especially important toward developing a stronger scientific foundation of these systems so that adequate regulatory decisions may be made. While there are many emerging methods available for the detection and characterization of these chemicals, nanotechnology has presented itself as a promising alternative toward creating more efficient assays. In particular, metallic nanoparticles and thin films exhibit unique optical properties that allow for highly sensitive and multiplexed studies to be performed. These plasmonic materials often preclude the use of molecular tags and labels, enabling direct characterizations and enhancing the throughput of biomolecular studies. However, their lack of specificity toward certain targets and potential toxicity has thus far precluded their widespread use in toxicity testing.
The cell membrane, a natural signal transducer, represents one of the fundamental structures for biological recognition and communication. These interfaces principally function as a selective barrier to exogenous materials, including ions, signaling molecules, growth factors, and toxins; therefore, understanding interactions at membrane interfaces is a vital step in elucidating how biological responses are effected. Supported lipid bilayers, which may easily be tailored in composition and complexity, are ideal interfaces for coupling to plasmonic assays since they may be supported in close proximity to metallic nanoparticles and thin films, where measurements are most sensitive. This research will focus on the coupling of plasmonic materials and biomimetic interfaces to increase the sensitivity, efficiency, and throughput of conventional toxicity assays. The fabrication of new plasmonic materials for membrane-based assays is presented, as well as method developments in membrane array formation and opportunities for hyphenation with complementary analytical techniques.
|Commitee:||Wang, Yinsheng, Zhong, Wenwan|
|School:||University of California, Riverside|
|School Location:||United States -- California|
|Source:||DAI-B 79/02(E), Dissertation Abstracts International|
|Subjects:||Analytical chemistry, Nanoscience, Materials science|
|Keywords:||Lipid membrane, Mass spectrometry, Microarray, Nanoscience, Supported lipid bilayer, Surface plasmon resonance|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be