The successful engineering of hydrogels with functionality, tunable mechanical properties, and degradation rates has led to significant advances in the field of tissue engineering. Depending on the chemical composition and the method of crosslinking, hydrogels vary in their morphology, network structure, mechanical properties, degradation behavior and biological activities. The major themes pursued in this thesis are the development of technology to facilitate the fabrication of hydrogels with controlled properties and functionality, the role of inorganic phase in the composite hydrogel system, and the study of the cellular response and cytocompatibility to our developed hydrogels. The present work has been organized into three parts. In the first part (Chapter 2-3), the hydrogels are prepared by amine-Michael type addition chemistry, and subsequently stabilized by means of additional photopolymerization to prolong the degradation time for cell test. The mechanical and degradation behavior of the gels have been adjusted in a controlled and tailorable fashion, and the functionality of gels is introduced, which allows biological motifs or soluble factors to be incorporated into the hydrogel matrices. Meanwhile, this step-growth polymerization method leads to long-ranged crystalline structure. The second part (Chapter 4) describes the in-situ formation of PEG-CP composite hydrogels, and the influence of inorganic CP phase on the gel properties. Cell adhesion test has confirmed the strong affinity between osteoblast and composite hydrogels containing CP phase, which is potentially used for the regeneration of bone tissue engineering. In the third part (Chapter 5), the hydrogel matrices with defined mechanical properties as well as tunable degradability have been created by click chemistry. Degradation time and the monitored mechanical properties of the hydrogels changed predictably as degradation proceeded until the gels reached complete degradation. These unique properties indicate that this degradable hydrogels are well suited for the applications in protein/cell delivery to repair soft tissue.
|School:||Technische Universitaet Berlin (Germany)|
|Source:||DAI-C 81/1(E), Dissertation Abstracts International|
|Subjects:||Inorganic chemistry, Materials science|
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