Dissertation/Thesis Abstract

A New Method of Genome-Scale Metabolic Model Validation for Biogeochemical Application
by Shapiro, Benjamin Morris, M.S., University of Oregon, 2017, 93; 10289762
Abstract (Summary)

We propose a new method to integrate genome-scale metabolic models into biogeochemical reaction modeling. This method predicts rates of microbial metabolisms by combining flux balance analysis (FBA) with microbial rate laws. We applied this new hybrid method to methanogenesis by Methanosarcina barkeri.

Our results show that the new method predicts well the progress of acetoclastic, methanol, and diauxic metabolism by M. barkeri. The hybrid method represents an improvement over dynamic FBA. We validated genome-scale metabolic models of Methanosarcina barkeri, Methanosarcina acetivorans, Geobacter metallireducens, Shewanella oneidensis, Shewanella putrefaciens and Shewanella sp. MR4 for application to biogeochemical modeling. FBA was used to predict the response of cell metabolism, and ATP and biomass yield. Our analysis provides improvements to these models for the purpose of applications to natural environments.

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Indexing (document details)
Advisor: Jin, Qusheng
Commitee: Bridgham, Scott, Davis, Edward, Watkins, Jim
School: University of Oregon
Department: Geological Sciences
School Location: United States -- Oregon
Source: MAI 56/06M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Geology, Biogeochemistry
Keywords: Biogeochemical reaction modeling, Flux balance analysis, Genome-scale, Geobacter, Methanosarcina, Shewanella
Publication Number: 10289762
ISBN: 9780355143669
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