Leaf senescence is the final stage of leaf development where older leaves undergo an active degenerative process. This highly coordinated event is characterized by a cascade of differential gene expression resulting in senescence upregulated and senescence downregulated genes. Cytosine methylation, a mechanism of epigenetic control, has been shown to play a role in regulating gene expression. Gene body cytosine methylation is correlated with transcriptional activation while promoter cytosine methylation is correlated with transcriptional repression. Evidence from previous work suggests CG methylation (mCG) in promoter regions plays a role in repressing gene expression and that a correlation between demethylation and mRNA induction is most likely within 500 bp up- and downstream of TSSs. The purpose of this study is to investigate the correlation between promoter cytosine methylation and transcriptional repression by identifying potential cytosine methylation-regulated senescence upregulated genes (CMR-SURGs). Four candidate CMR-SURGs were identified from previously generated RNA-seq data and an online cytosine methylome. We hypothesized that the four CMR-SURGs would display a correlation between mRNA induction and loss of promoter mCG. mRNA expression was measured by real-time qPCR, and cytosine methylation was quantified by bisulfite treatment of genomic DNA followed by PCR, cloning, and sequencing of PCR products. These data however, showed that only UGT78D1 displayed a negative correlation between promoter cytosine methylation and age-related mRNA induction.
|Commitee:||Pace, Douglas, Tsai, Houng-Wei|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 56/06M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Genetics, Plant sciences|
|Keywords:||Arabidopsis, Cytosine methylation, Epigenetics, Senescence, Surg, Ugt78d1|
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