Fibroblast growth factors (FGFs) and their receptors (FGFRs) play a vital part in the proper development and maintenance of the brain. FGFR1, which is one of four FGFRs total and one of three found in the brain (FGFR1-3), has been shown to be important in cellular proliferation, cellular migration, synaptogenesis, cellular morphology, and has also been implicated in multiple neuropsychiatric disorders. Understating the role FGFR1plays in these and other cellular processes is vital to our understanding of the human body and in the prevention and treatment of some neuropsychiatric and developmental disorders. Although previous studies have produced groundbreaking findings in the field, they have fallen short in the accurate identification of which cell type express Fgfr1. Therefore, to validate the use of a transgenic mouse line in the accurate and efficient study of Fgfr1 expression during experimental manipulations and cellular localization, we utilized the tgFGFR1- EGFPGP338Gsat BAC mouse line (tgFgfr1-EGFP+) obtained from the GENSAT project. By utilizing the tgFgfr1-EGFP+ mouse line, we were able to accurately identify which cell types in the embryonic and perinatal mouse brain express Fgfr1. Furthermore, we were able to measure relative changes in Fgfr1 expression via GFP fluorescence as a proxy after both exposure to chronic stress and the chemical demyelinator, cuprizone. The combination of our results lead us to conclude that the tgFgfr1-EGFP+ mouse line is a very useful tool in the study of FGFR1 and may aid in the identification of potential targets for therapeutic treatment of the many disorders associated with FGFR1 signaling.
|Advisor:||Smith, Karen M.|
|Commitee:||Kulkarni, Ritwij, Watson, Glen|
|School:||University of Louisiana at Lafayette|
|School Location:||United States -- Louisiana|
|Source:||MAI 56/06M(E), Masters Abstracts International|
|Subjects:||Neurosciences, Cellular biology, Developmental biology|
|Keywords:||Blbp, Fgf, Fgfr1, Fibroblast growth factor receptor 1, Fibroblast growth factors|
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