Dissertation/Thesis Abstract

The author has requested that access to this graduate work be delayed until 2018-09-16. After this date, this graduate work will be available on an open access basis.
Development and Characterization of Histidinetagged HPV16 L2 and MS2-Argininetagged Recombinant Proteins for Downstream Processes
by Alothaim, Tahiyat, M.S., Michigan Technological University, 2017, 54; 10604258
Abstract (Summary)

Human papillomaviruses (HPVs) are the most common sexually transmitted infections. Persistent infection with HPV can lead to anogenital cancers including head and neck cancers. Three prophylactic vaccines have been approved to prevent against some types of HPV infection. However, the vaccines are HPV-type specific and protect mostly against the HPV types included in the vaccines. To offer broader protection against more HPV types, studies in the field are developing candidate vaccines targeting a conserved minor capsid protein, L2. Nevertheless, reagents for developing and assessing L2 vaccines are limited. For example, antibodies to assess the antigenicity of some L2 epitopes are not available commercially and multivalent platforms to develop and purify clinical grade L2 antigens are limited. In this study, I developed and characterized the immunogenicity of a recombinant Histidine-tagged HPV16 L2 (amino acid 1-130) antigen. In addition to this, I explored the development of a multivalent display platform, a recombinant MS2-bacteriophage coat protein, with a C-terminal Arginine-tag for downstream purification using cation exchange chromatography. All Recombinant proteins (Histidine-tagged L2 and MS2-Arg tagged) were successfully expressed in bacteria. However, only Histidine-tagged L2 proteins were successfully purified in large quantity to homogeneity. Mice immunized with the Histidine-tagged L2 protein elicited anti-L2 IgG antibody titers greater than 103. The anti-L2 antibodies generated in this study will be valuable to researchers, in the field, developing L2 vaccines.

Indexing (document details)
Advisor: Tumban, Ebenezer
Commitee: Joshi, Chandrashekhar, Techtmann, Stephen
School: Michigan Technological University
Department: Biological Sciences
School Location: United States -- Michigan
Source: MAI 56/06M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Biology, Genetics, Biochemistry
Keywords: Diseases, Genetics and genomics, Microbiology, Mmunology and infectious disease, Vaccine, Virology
Publication Number: 10604258
ISBN: 9780355316247