Dissertation/Thesis Abstract

The author has requested that access to this graduate work be delayed until 2019-06-21. After this date, this graduate work will be available on an open access basis.
Dynamic Genetic Control: Manipulating Cell Behaviors Using Heat
by Ji, Yanmin, M.S., University of California, San Diego, 2017, 36; 10287163
Abstract (Summary)

Although Chimeric Antigen Receptor (CAR) T cells have been proven to be powerful in eradicating tumors. However, its non-specific activity against normal tissues and cells has been a major problem. Here, utilizing the activation of transient receptor potential cation channel subfamily V member 1 (TRPV1) under heat, the translocation of nuclear factor of activated T-cells (NFAT) triggered by Calcium influx, and the recombination effects of Cre-lox system, we have developed a system to remotely manipulate the expression of proteins in cells under the control of both heat and drugs. The significance of this project is to provide a method to precisely control the activities of CAR T cells within targeted local tissues.

Indexing (document details)
Advisor: Wang, Yingxiao
Commitee: Hao, Nan, Mali, Prashant
School: University of California, San Diego
Department: Bioengineering
School Location: United States -- California
Source: MAI 56/05M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Molecular biology, Biomedical engineering
Keywords: Cre, FKBP/FRB, Heat, Short exposure, TRPV1
Publication Number: 10287163
ISBN: 9780355058239