Large intracranial arteries are unique compared to conduit vessels in the extracranial vasculature. They differ in resistance, blood flow regulation, reactivity to vasostimuli, and the accumulation of atherosclerotic plaque formation. Large cerebral vessels are atheroprotective, while conduits are atheroprone. Central to the observed differences are the vascular smooth muscle cells (VSMCs). Unique transcriptional activity in cerebral VSMCs may be responsible for the atheroprotective nature of the intracranial vasculature. Krüppel-Like Factor (KLF) 4 helps promote cellular de-differentiation, while KLF5 facilitates proliferation. Working together, the two KLFs may facilitate the early onset of atherosclerosis in the aorta, relative to the cerebral vasculature. The current study aimed to address whether or not those KLFs were partially responsible for the observed differences in the two types of arteries. Laser microdissection was used to isolate VSMCs from the aorta and basilar artery to measure KLF abundance using rt-qPCR.
|Advisor:||Kolega, John, Sim, Fraser|
|School:||State University of New York at Buffalo|
|School Location:||United States -- New York|
|Source:||MAI 56/05M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Biochemistry|
|Keywords:||Atherosclerosis, Conduit vessel, Kruppel-like factor, Large cerebral vessel, Microdissection, Vascular smooth muscle cell|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be