Cardiovascular disease continues to be the leading cause of mortality and morbidity in the United States. Current treatment options are aimed at preventing additional injury and helping the heart work more efficiently, but are limited in their regenerative capacity. Recently, research has shown that treating the heart with various stem cell populations, including cardiosphere-derived cells (CDCs), post myocardial infarction (MI) stimulates regeneration, angiogenesis, and functional improvement. While this treatment has shown promise in early stage clinical trials, there remains a gap in the ability to efficiently deliver tissue-specific agents directly to the heart while avoiding nonspecific delivery to other organs. To fully realize the therapeutic potential of efficient delivery to the heart, we engineered CDC-derived exosomes (nano-vesicles that transport RNA and protein between cells) to express Lamp-2b, an exosomal trans-membrane protein, fused with a cardiomyocyte-specific peptide. Preliminary experiments showed enhanced exosome uptake by cardiomyocytes in vitro, establishing a novel tool for targeted delivery of anti-apoptotic drug and gene therapy.
|Advisor:||Lang, Jennifer K., Sarkar, Debanjan|
|School:||State University of New York at Buffalo|
|School Location:||United States -- New York|
|Source:||MAI 56/05M(E), Masters Abstracts International|
|Subjects:||Biomedical engineering, Medicine|
|Keywords:||Cardiomyocytes, Cloning, Exosomes, Localization, Targeted delivery|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be