Schizophyllum commune is a basidiomycete species that has an obligate heterothallic mating system by which it achieves sexual reproduction. Upon mating the mating-type gene products activate downstream molecular events, which lead to dikaryotization, fruiting, and sporulation. Small cysteine-rich proteins called hydrophobins are among the many proteins that are developmentally regulated in the process. The SC4, SC1, and SC6 hydrophobins are tightly controlled and are considered “dikaryon-specific”. The SC3 hydrophobin aids in formation of aerial hyphae and attachment to hydrophobic surfaces, and is strongly expressed in unmated tissues. Schizophyllum umbrinum is a lesser studied species in the genus Schizophyllum. It exhibits a homothallic life cycle, has no regular pattern of dikaryotic hyphae, but produces fertile fruiting bodies. We found that culturing S. umbrinum on potato flake media improved the speed and luxuriance of mushroom production compared to other media. A 37-day timeline of development for cultured S. umbrinum was produced from the observations. Hydrophobins were anticipated to exist in S. umbrinum, and were predicted show a regulated expression pattern that could be compared to S. commune to better understand the homothallic development of S. umbrinum. Hydrophobin sequences from several basidiomycetes were aligned to identify conserved positions for PCR primer design. Polymerase Chain Reaction (PCR), Southern and northern blots, as well as a small scale cDNA library screen failed to clearly identify hydrophobin genes from S. umbrinum or their expression. A 4.5 kilobase DNA restriction fragment in genomic DNA from S. umbrinum resulted in the strongest signal to an S. commune SC4 probe.
|Advisor:||Fowler, Thomas J.|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 56/05M(E), Masters Abstracts International|
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