Target of Rapamycin (TOR) Complex 2 (TORC2) is a conserved multi-subunit protein kinase associated with the plasma membrane that is an essential regulator of growth. In Saccharomyces cerevisiae, TORC2 regulates the lipid composition and organization of the plasma membrane during normal cell growth and, in turn, responds to environmental insults (such as changes in osmotic conditions) that exert stress on the plasma membrane to maintain homeostasis. Ample genetic and biochemical evidence indicates that TORC2 exerts its effects solely via direct phosphorylation and stimulation of the activity of the downstream protein kinase Ypk1 (and its paralog Ypk2). Ypk1 action modulates plasma membrane lipid homeostasis in multiple ways, including up-regulation of sphingolipid synthesis and inhibition of aminoglycero-phospholipid flipping. Ypk1 also controls glycerol production and efflux, allowing cells to adapt to osmotic changes. Prior work demonstrated that TORC2 phosphorylates Ypk1 at two conserved sequence elements near its C-terminus, dubbed the "turn" and "hydrophobic" motifs. However, this study documents that TORC2 also phosphorylates Ypk1 at four additional C-terminal sites that are also critical for full TORC2-mediated stimulation of Ypk1 activity. Ala substitutions at the four new sites abrogated the ability of Ypk1 to rescue the phenotypes of Ypk1 deficiency, whereas Glu substitutions had no ill effect. Combining the Ala substitutions with an N-terminal mutation (D242A) that has been shown to bypass the need for TORC2 phosphorylation restored the ability to complement a Ypk1-deficient cell. These findings provide new insights about the molecular basis for TORC2-mediated activation of Ypk1. Moreover, TORC2 phosphorylation of Ypk1 changes differentially in response to different plasma membrane stresses; it is elevated in a sustained manner upon sphingolipid depletion, but rapidly and greatly diminished, although only transiently, upon hyperosmotic shock. In this work, new insights were also obtained about how hypertonic conditions influence TORC2. Results described here document that the plasma membrane osmosensor Sln1 is an upstream regulator of TORC2. Inactivation of Sln1, which causes activation of the Hog1 MAPK, leads to loss of TORC2 phosphorylation of Ypk1. This response requires the Hog1 MAPK itself and also the Slt2 MAPK. Upon Sln1 inactivation, Avo2 is hyperphosphorylated at its MAPK phosphoacceptor sites in a Hog1 and Slt2-dependent manner. These findings suggest that MAPK-mediated phosphorylation of Avo2 may provide a mechanism for exerting negative regulation on TORC2 function.
|Advisor:||Thorner, Jeremy W.|
|Commitee:||Drubin, David G., Dueber, John E., Hurley, James H.|
|School:||University of California, Berkeley|
|Department:||Molecular and Cell Biology|
|School Location:||United States -- California|
|Source:||DAI-B 78/11(E), Dissertation Abstracts International|
|Subjects:||Molecular biology, Cellular biology, Biochemistry|
|Keywords:||Rapamycin, TORC2, Ypk1|
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