Photosynthetic complex assembly within the plastid is a blossoming field as science uncovers additional requirements and levels of regulation in the production of these protein complexes. Using both forward and reverse genetics, our lab has revealed two new players in the assembly of Photosystem II and the b6f complex. The first of these factors is LTO1, identified as the thiol oxidant for the production of a disulfide bond within PsbO, a PSII luminal protein. The second is an OPR protein with a novel role in the attachment of heme to cytochrome f of the b6f complex. The presence of these assembly factors opens exciting new doors in the possibilities of plastid b, as demonstrated by our relocation of the nuclear ARG9 gene into the plastid genome.
|Commitee:||Bird, Amanda, Mackey, Dave, Somers, Dave|
|School:||The Ohio State University|
|Department:||Plant Cellular and Molecular Biology|
|School Location:||United States -- Ohio|
|Source:||DAI-B 78/11(E), Dissertation Abstracts International|
|Keywords:||ARG9, CCS2, Cytochrome, Dithiol oxidase, LTO1, Lumen|
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