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Dissertation/Thesis Abstract

Construction and Senescence Phenotype Analysis of Double Mutants Encoding H3K4me3 Methyltransferases in Arabidopsis thaliana
by Barman, Soumi, M.S., California State University, Long Beach, 2017, 74; 10257592
Abstract (Summary)

Leaf senescence, which is the final process of leaf development, involves a complex regulation of thousands of genes to recover and recycle valuable nutrients and mobilize them to growing part of the plant for high yield of fruits and grains. A greater understanding of the complex senescence gene regulation could be helpful for higher crop yield. This study is focused on three genes (ATX1, ATX3 and ATX4) that code for H3K4me3 methyl transferases to investigate their effect on flowering transition time, and their importance during senescence by assaying total chlorophyll and protein levels, and quantifying the mRNA expression of senescence marker gene WRKY75. An additive early flowering phenotype was observed for double mutants. However, no senescence alteration was found for double mutants. An increased level of total chlorophyll was shown by single mutant atx4. Significant differences for total protein were observed in leaf 6 vs. leaf 7 for double mutants atx1atx3 and atx1atx4, suggesting a faster protein degradation rate or smaller variability (reduced confidence interval) in leaf 7 data. Due to the gene redundancy of the ten-member ATX family, knocking out two genes may not adequately affect the function of H3K4 methyltransferase activity. Therefore, phenotypic analyses of triple and quadruple mutants of senescence-expressed H3K4me3 methyltransferase coding genes may show stronger senescence phenotypes. Of importance, these data show that significantly early flowering does not dictate early leaf senescence.

Indexing (document details)
Advisor: Brusslan, Judy
Commitee: Eldon, Elizabeth, Fraser, Deborah
School: California State University, Long Beach
Department: Biological Sciences
School Location: United States -- California
Source: MAI 56/04M(E), Masters Abstracts International
Subjects: Biology
Keywords: Arabidopsis, H3K4me3, Methyltransferases, Mutants, Phenotype, Senescence
Publication Number: 10257592
ISBN: 978-1-369-86167-9
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